A modular, self-paced, mobile-friendly online course on foundational infection prevention and control (IPC) was developed for health workers (HWs) in Africa, addressing challenges in accessing training. Evaluated in Nigeria, the course comprised 10 modules utilizing IPC vignettes and short assessment questions. Of the 372 learners who enrolled, 59% completed all modules, demonstrating significant pre-test (29%) to post-test (54%) score improvements (effect size 1.22). User experience was rated positively, with a Net Promoter Score of +62. These results suggest that scalable online training can effectively bridge IPC knowledge gaps among HWs in the region.